UCT HYDROPHOBIC EXTRACTION COLUMNS | Chromatography Direct
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HYDROPHOBIC EXTRACTION COLUMNS

This sorbent is composed of a silica backbone bonded with hydrocarbon chains. It is used to extract compounds which exhibit non-polar or neutral characteristics out of complex matrices. The C18 phase is the most widely used for non-polar interactions because of its non-selective nature; C18 will extract a large number of compounds with differing chemical properties. To enhance selectivity, UCT offers a wide range of hydrophobic sorbents. Multiple chain configurations are available for some End capped or un end capped sorbents are available for all chain lengths.

Compounds are retained on the sorbent through ionic bonds. Therefore, it is essential that the sorbent and the analyte to be extracted are charged. Generally, the number of molecules with charged cationic groups increases at pH values below the molecules pKa value. The number of molecules with charged anionic groups decreases at pH values below the molecule’s pKa value. To ensure 99% or more ionization, the pH should be at least two pH units below the pKa of the cation and two pH units above the pKa of the anion. Elution occurs by using a solvent to raise the pH above the pKa of the cationic group or to lower the pH below the pKa of the anion to disrupt retention. At this point, the sorbent or compound will be neutralized.
Carbon supports have been used to isolate extremely polar organic compounds. They work by an adsorption mechanism with a high surface area and ion exchange.
These sorbents are composed of a silica backbone bonded with a carbon chain terminated by a negatively charged functional group. UCT cation exchange sorbents provide unmatched activity for amines, alcohols and other nucleophiles. Our extensive product line allows the analyst to choose from a wide range of cartridge sizes and chemistries each tailored to the specific requirements of the extraction.
This sorbent is composed of a silica backbone with two types of functional chains attached - an ion exchanger or polar chain and a hydrophobic carbon chain. Our copolymeric phases are produced in a way to allow for equal parts of each functional group to attach to the silica backbone. This copolymerization technique yields reproducible bonded phases and unique copolymeric chemistries which allow the controlled use of mixed mode separation mechanisms. This type of dual chemistry is beneficial especially when one is looking for both a neutral & charged compound. This is common when a neutral parent drug metabolizes & becomes a charged compound.
Covalent sorbent has aldehyde functional groups that are bound to the silica backbone by a hydrocarbon chain. The aldehyde group will react selectively with compounds containing a primary amine. A formal bond is created between the stationary support and the primary amine containing material.The primary amine in the sample performs a nucleophilic attack on the aldehyde functional group attached to the support. This results in a Schiff base, with the amine immobilized on the stationary support. This chemistry can be utilized to bind proteins, such as antibodies, to the support, allowing highly specific extractions.

Clean-Up® Hydrophilic
This sorbent is composed of a silica backbone bonded with carbon chains containing polar functional groups. Groups which will possess such polarity include amines, hydroxyls and carbonyls.Compounds are retained on hydrophilic sorbents through polar interactions including hydrogen bonding, pi-pi or dipole-dipole interaction. These types of interactions occur when a distribution of electrons between individual atoms in functional groups is unequal, causing negative and positive polarity. Compounds typically extracted on a hydrophilic column include analytes which have polar groups, including amines, hydroxyls and carbonyls. Elution is performed by strong polar solvents.

Clean-Up® Hydrophobic
This sorbent is composed of a silica backbone bonded with hydrocarbon chains. It is used to extract compounds which exhibit non-polar or neutral characteristics out of complex matrices. The C18 phase is the most widely used for non-polar interactions because of its nonselective nature; C18 will extract a large number of compounds with differing chemical properties. To enhance selectivity, UCT offers a wide range of hydrophobic sorbents, from C2 to C20. Multiple chain configurations are available for some sorbents. Endcapped or unendcapped sorbents are available for all chain lengths.