UCT Clean-Up Covalent Extraction Columns | Chromatography Direct
Sign InRegister

Partnumber Search

Website Sign In

Clean-Up Covalent Extraction Columns

Covalent sorbent has aldehyde functional groups that are bound to the silica backbone by a hydrocarbon chain. The aldehyde group will react selectively with compounds containing a primary amine. A formal bond is created between the stationary support and the primary amine containing material.The primary amine in the sample performs a nucleophilic attack on the aldehyde functional group attached to the support. This results in a Schiff base, with the amine immobilized on the stationary support. This chemistry can be utilized to bind proteins, such as antibodies, to the support, allowing highly specific extractions.